SDS法,SDS method
1)SDS methodSDS法
1.aconiti by usingSDS method,CTAB method and the modified CTAB method resp.[方法]以中药材附子药源植物的幼嫩叶片和块根为试材,分别采用SDS法、CTAB法和改良CTAB法从中药材附子药源植物中提取基因组DNA,比较不同方法的提取效果。
2.This research reported a developmentSDS method for extraction cotton genomic DNA.通过在目前常用的SDS法的DNA提取液中加入抗氧化剂(PVP40和β-巯基乙醇)排除棉酚等次生物质干扰,进行了棉花基因组DNA的提取方法研究,结果表明,改良后的SDS法能够解决棉花DNA提取质量不高且容易褐变等问题。
3.To probe the adaptability of modifiedSDS method in apricot genomic DNA extraction and the optimal concentration, the genomic DNA samples of 6 apricot cultivars were isolated by 0.为探讨改良SDS法是否适用于杏基因组DNA提取及提取液适宜浓度,以6个品种杏叶片为试材提取DNA,提取液设0。
英文短句/例句
1.DNA Extraction from Chimonanthus praecox by CTAB and SDSSDS法和CTAB法提取蜡梅DNA质量的比较
2.Extraction of Genomic DNA of Dendrobium with SDSSDS法提取石斛基因组DNA的研究
3.Study on Pyrrosia Genomic DNA Extraction by Modified SDS Method改良SDS法提取石韦基因组DNA的研究
4.Detection of Heating Endpoint Temperature for Fish by SDS-PAGE MethodSDS-PAGE电泳法检测鱼肉热处理终点温度
5.Recovery and Reuse of SDS by Ultrafiltration with Acid or Chelator酸化超滤法和螯合超滤法回收再利用SDS胶团
6.Interaction between sodium alginate and sodium dodecyl sulfate probed by pyrene fluorescence芘荧光探针法研究海藻酸钠与SDS的作用
7.Determination of Critical Micellar Aggregation Numbers of SDS by Steady-state Flourescence Probe Method稳态荧光探针法测定SDS表面活性剂胶束聚集数
8.A Modified Method for SDS-PAGE StainingSDS-聚丙烯酰胺凝胶电泳染色方法的改进
9.Leaf Protein Extraction Methods Suitable for SDS-PAGE Analysis in Apple适于SDS-PAGE分析的苹果叶片蛋白质提取方法
10.Determination of protein by resonance rayleigh light scattering technique utilizing lumogallion-SDS as probe荧光镓-SDS共振瑞利散射法测定蛋白质的研究
11.Determination of Amikin with Eosin Y by Resonance Light Scattering Method Using SDS as Sensitizer曙红Y-SDS体系共振光散射法测定丁胺卡那霉素
12.Polyelectrolyte as Vehicles for Isolation and Purification of Alkalescence Protein and the Conditions Optimization of Alkalescence Peptides SDS-PAGE;高分子电解质分离碱性蛋白的方法及碱性多肽SDS-PAGE条件的优化
13.Research on the Methods of Determining the Chemical Elements Content in Pyrotechnic Composition on the Safety Data Sheet烟花爆竹安全数据表（SDS）中各化学组分含量测定方法研究
14.A Facile One-pot Synthesis of Hollow Cu_2O Submicrospheres in Virtue of Template Function of the SDS-PVP Necklace-like Soft Clusters以SDS-PVP项链状软团簇为模板简易一锅法合成Cu_2O中空亚微球
15.Determination of protein by resonance light scattering method after combining with falcon carboxylic acid SDS钙指示剂羧酸钠-SDS体系共振瑞利散射法测定蛋白质
16.SDS-PAGE methods for comparative study of the heat-stable water-soluble proteins from barley and maltSDS-PAGE方法比较研究大麦及麦芽中热稳定性水溶蛋白
17.Application of Three Kinds of Staining Methods for PEGylated Protein System in SDS-PAGE聚乙二醇修饰蛋白体系的SDS-聚丙烯酰胺凝胶电泳染色方法新探
18.An Improved SDS Acid Phenol Method for Total RNA Extraction from Plant Tissues Rich in Lipid一种提取富含油脂植物组织总RNA的改良SDS酸酚法
相关短句/例句
SDSSDS法
1.We adopted the phenol-chloroform-isopentanol, CTAB andSDS methods to extract DNA from Sinononacula constricta , Meretix linnaeus,Chlamys nobilis, Haliotis diversicolor and other shellfishes.以缢蛏、美人蛏、文蛤、扇贝和鲍鱼等几种经济贝类为研究对象,分别用苯酚/氯仿/异戊醇法,CTAB法,SDS法等不同方法提取其基因组DNA,寻找适宜于研究对象基因组DNA提取的方法,结果表明,3种方法均可以从目标材料中提取到DNA,但苯酚/氯仿/异戊醇法无论从数量和质量上都不理想,而SDS和CTAB可根据具体情况选择使用。
2.The results showed thatSDS-CTAB method was the best one,thenSDS method,and CTAB method was not recommended.以籽蒿(Artemisia sphaerocephala Krasch)种子为试验材料,采用SDS法、CTAB法及SDS-CTAB法进行基因组DNA提取。
3.The tota1 DNA was extracted from the bean,horse bean and soybean with methods of high salt,SDS and CTAB.以菜豆、蚕豆、黄豆的幼嫩叶片及其种子为材料,采用高盐低pH值法、SDS法和改良CTAB法3种不同的DNA提取方法提取其总DNA,用紫外分光光度法和琼脂糖凝胶电泳检测DNA质量。
3)sodium dodecylsulphate-polyacrylamide gel electrophoresisSDS-PAGE法
1.Methods Gel filtration chromatography(GFC),sodium dodecylsulphate-polyacrylamide gel electrophoresis(SDS-PAGE) technique and matrix-assisted laser desorption/ionizationtime of flight mass spectrometry(MALDI-TOF-MS)were used to determine the relative molecular weight,respectively.方法采用凝胶过滤层析法、SDS-PAGE法和基质辅助激光解吸附电离飞行时间质谱法(MALDI-TOF)三种方法测定一种新型蚯蚓脱氧核糖核酸酶(EWD2)的分子量。
4)borax-SDS硼砂-SDS法
1.In order to isolate high quality RNA from young leaves of Pyrus ussuriensis Maxim,in which polyphenols and polysaccharide are rich,The authors used five methods as follows: Trizol reagent;improved Trizol reagent;Guanidine Thiocyanate;borax-CTAB;borax-SDS,and established the most suitable isolation method of Pyrus ussuriensis Maxim RNA through the rate of yield,purity,electrophoresis and RT-PCR.为了从富含多糖和多酚等物质的观赏山梨幼叶中提取和分离出高质量的RNA,利用Trizol法、改良的Trizol法、异硫氰酸胍法、硼砂-CTAB法和硼砂-SDS法提取观赏山梨叶片总RNA。
5)improved SDS method改良SDS法
1.Method: The improved CTAB method andimproved SDS method were used to extract DNA.方法:采用改良CTAB法和改良SDS法提取澳洲坚果样品的总DNA,并对产物进行紫外、电泳及PCR扩增检测。
2.Traditional SDS method was simplified and improved,improved SDS method was found,DNA which was extracted from root tips of peanut byimproved SDS method was high quality and was satisfied for molecular biology research.对传统的SDS法进行简化和优化,建立了改良SDS法,利用改良SDS法提取花生根尖DNA,效果较好,能够满足分子生物学研究需要。
6)SDS-CTAB methodSDS-CTAB法
1.Results: Tilletia caries teliospores DNA of this year,purity(OD260/OD280):CTAB method>SDS-CTAB method>SDS method>urea method=1.方法:应用改良过的四种方法(CTAB法、SDS-CTAB法、SDS法和尿素法)对小麦网腥黑粉菌冬孢子总DNA进行提取。
延伸阅读
akyposal sdsCAS: 151-21-3 分子式: C12H25NaO4S 分子质量:288.38 熔点: 206℃ 中文名称: 十二烷基硫酸钠；十二烷基硫酸酯钠盐；十二醇硫酸钠；月桂醇硫酸钠；发泡粉 英文名称: Sodium dodecyl sulfate；Sulfuric acid monododecyl ester, sodium salt；Sodium lauryl sulfate；akyposal sds；aquarex me；aquarex methyl； sulfuricacid,monododecylestersodiumsalt；Sodium dodecanol sulfate性质描述: 白色至微黄色粉末。熔点180-185℃（分解）。易溶于水而成半透明溶液，对碱；弱酸和硬水都很稳定。微有特殊气味。 生产方法: 将十二醇与氯磺酸按摩尔比1：1.03投料，在30-35℃进行磺化反应，生成的磺酸酯用30%氢氧化钠中和，生成十二醇硫酸钠，经双氧水漂白；喷雾干燥即得成品。原料消耗（kg、t）月桂醇（C12>85%） 725固碱（NaOH>95%） 183氯磺酸（>95%） 458 用途: 用作洗涤和纺织助剂，也用作牙膏发泡剂，灭火泡沫液，乳液聚合乳化剂，医药用乳化分散剂，洗发剂等化妆制品，羊毛净洗剂。
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